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. 2008 Oct 28;99(11):1891–1899. doi: 10.1038/sj.bjc.6604754

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Copyright © 2008 Cancer Research UK

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(A) Diagram of the IGF2 P3 promoter region. Individual CpG dinucleotides located upstream of exon 6 (from −1068 to −702 bp) are represented by circles. Horizontal arrows indicate locations of PCR primers used for MSP and bisulphite sequencing. (B) Examples of the promoter methylation status using methylation-specific PCR. Polymerase chain reaction products of methylated or unmethylated P3 promoters from HB tumours are shown. Numbers above horizontal bars indicate the tumour number. M, methylated promoter; U, unmethylated promoter. (C) Bisulphite sequencing analysis of the methylation status of P3 promoter in HuH6 and one tumour (no. 1), which displayed complete methylation and complete unmethylation, respectively. Open and closed circles indicate unmethylated and methylated CpG dinucleotides, respectively. (D) Levels of P3 transcripts in tumours with partially methylated P3 promoter and tumours with unmethylated P3 promoter.