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. 2008 Nov 11;99(11):1832–1841. doi: 10.1038/sj.bjc.6604763

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Copyright © 2008 Cancer Research UK

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Inhibition of cell invasion by γ-T3 treatment. (A) 24-h dose-dependent and IC₅₀ time-dependent γ-T3 treatment induces the expression of epithelial markers (E-cadherin, γ-catenin), but suppresses the expression of mesenchymal markers (vimentin, twist and α-SMA) and E-cadherin's repressor (snail). (B) The invasive androgen-independent PCa cells (PC-3) treated with the indicated dosage of γ-T3 was harvested and then plated into the Matrigel-coated (0.5 mg ml⁻¹) insert. Cells invaded through the membrane were stained with crystal violet and the images were photographed under a microscope. After being lysed with extraction buffer, intensity at 595 nm was measured.