Figure 5.

Synergistic effect of γ-T3 on Docetaxel-induced apoptosis. (A) Effect of Docetaxel and γ-T3 co-treatment for 24-h. Cells were incubated with different dosages of γ-T3 and 100 nM of Docetaxel for 24 h. Cell viability was examined by MTT assay. The percentage of apoptotic PC-3 and LNCaP cells following co-treatment of Docetaxel and γ-T3 was significantly higher than that treated with either agent alone. (B) Using western blotting, we further demonstrated that γ-T3 co-treatment with Docetaxel for 24-h enhances PC-3 cell apoptosis through activation of pro-apoptotic molecules (cleaved PARP, caspases 3, 7, 8, 9). Additional suppression of proliferation genes were also confirmed for Id-1, EGF-R, iκB, and NF-κB p65. (C) Proposed T3 anticancer pathway in PCa cells.