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. 2008 Nov 11;99(11):1849–1858. doi: 10.1038/sj.bjc.6604774

Figure 4.

Figure 4

The effects of siRNA-mediated knockdown of TEAD1 or c-Cbl in RWPE1 and PC3 cell lines. RWPE1 and PC3 cells were reverse transfected using two siRNA oligos per target gene. Mock transfection (M) and non-targeting siRNA (NT) were controls. (A) MTS proliferation assays were performed over 96 h post-transfection. Proliferation was normalised to that of the non-targeting control. Mean of three experiments, bars=s.e.m.; *P<0.05; ***P<0.01. Representative western blots at 72 h post-transfection are shown. (B) Transfected RWPE1 cells were grown in Matrigel for 4 days and the % failure for the formation of spherically polarised acini was calculated. Mean of three experiments, bars=s.e.m. Knockdown of TEAD1 and c-Cbl significantly increased the failure rate (P-values; T1_1=0.006, T1_3=0.009; cbl_8=0.08, cbl_9=0.003). Phase-contrast microscopy images of spheres: A=non-targeting control, B=T1_1 and C=cbl_9. Original magnification × 40, bar=50 μm. The level of knockdown remaining after 4 days was confirmed by western blotting. Representative blots for the three experiments are shown.