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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Exp Hematol. 2008 Sep 23;36(12):1585–1592. doi: 10.1016/j.exphem.2008.07.001

Figure 3. miR150 affects a c-Myb 3′ UTR reporter gene.

Figure 3

Figure 3

Figure 3

A. The pCMV-luc-3′UTRc-Myb reporter gene is illustrated [with the list of miR binding sites]. The construct was generated by introducing the 1.2 kb 3′UTR of c-Myb into pMIR-Report-luc. B. pCMV-luc-3′UTRc-Myb was transfected into UT7/TPO cells with miR150, miR195 or a negative miR control. After 24 hr, luciferase activity was assessed in duplicate cultures. Values reported are the mean ± S.E.M. of three separate experiments and reported as relative luciferase activity between the various conditions. The same vector, but without the 3′UTR of c-myb (pCMV-luc), were also utilized as controls, and results are shown. C. pCMV-luc-3′UTRc-Myb was transfected into UT7/TPO cells with anti-miR150, anti-miR195 or a negative anti-miR control. After 24 hr luciferase activity was assessed in duplicate cultures and is reported as the mean ± S.E.M. of three separate experiments. The same controls as in figure 3B were performed. pCMV-luc-3′UTRc-Myb was also transfected into the cells with miR negative control and anti-miR negative control, and these gave the same values as the vector without the 3′UTRcMyb (data not shown).