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. 2008 Dec 15;183(6):1075–1088. doi: 10.1083/jcb.200806035

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© 2008 Grossmann et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Can1 is released from MCC patches before endocytosis. (A) Can1-GFP was localized in the wild-type (WT), nce102Δ, and pil1Δ cells before (top) and 90 min after the addition of 5 mM arginine (middle). Arginine-induced loss of patchy Can1-GFP pattern on the surface confocal sections (left) and the amount of the internalized protein on transversal sections could be easily followed. Note the significantly more intensive vacuolar staining in the mutants lacking the MCC patches as compared with wild type. The whole experiment is documented in Fig. S3 (available at http://www.jcb.org/cgi/content/full/jcb.200806035/DC1). (B) Extractability of Can1-GFP in Triton X-100 was detected in the membranes of wild-type cells before and 10 min after the addition of 5 mM arginine. Anti-GFP antibody was used for the detection of the protein on Western blots. Bar, 5 μm.