Table I. Members of MCC and associated cytosolic proteins.

Name	ORF	Molecular function	Biological process	TMDs	Localization references
Integral MCC     components
Can1	YEL063C	H+-driven arginine permease	Basic amino acid transport	12	Malínská et al., 2003
Fur4	YBR021W	H+-driven uracil permease	Uracil transport	12	Malínská et al., 2004
Tat2	YOL020W	H+-driven tryptophan and tyrosine permease	Aromatic amino acid transport	12	Grossmann et al., 2007
Nce102	YPR149W	Unknown	Nonclassical protein secretion	4	This study
Fmp45	YDL222C	Unknown	Ascospore formation	4	Young et al., 2002
Sur7	YML052W	Unknown	Ascospore formation	4	Malínská et al., 2003; Young et al., 2002
Ygr131w	YGR131W	Unknown	Unknown	4	This study
Ylr414c	YLR414C	Unknown	Unknown	4	This study
Ynl194c	YNL194C	Unknown	Ascospore formation	4	Young et al., 2002
MCC-associated     cytosolic proteins
Lsp1	YPL004C	Protein kinase inhibitor activity	Endocytosis (eisosome)	0	Walther et al., 2006
Mdg1	YNL173C	Unknown	Pheromone signaling	0	This study
Pil1	YGR086C	Protein kinase inhibitor activity	Endocytosis (eisosome)	0	Walther et al., 2006
Pkh1	YDR490C	Ser/Thr protein kinase	Endocytosis	0	Roelants et al., 2002; Walther et al., 2007; Luo et al., 2008
Pkh2	YOL100W	Ser/Thr protein kinase	Endocytosis	0	Roelants et al., 2002; Walther et al., 2007; Luo et al., 2008
Pst2	YDR032C	Unknown	Unknown	0	This study
Rfs1	YBR052C	Unknown	Unknown	0	This study
Slm1	YIL105C	Phosphoinositide binding	Actin cytoskeleton organization	0	This study; Fadri et al., 2005
Slm2	YNL047C	Phosphoinositide binding	Actin cytoskeleton organization	0	Fadri et al., 2005
Ycp4	YCR004C	Unknown	Unknown	0	This study
Ygr130c	YGR130C	Unknown	Unknown	0	This study
Ymr031c	YMR031C	Unknown	Unknown	0	This study

TMD, transmembrane domain. Putative transmembrane domains are given as predicted by TmPred and transmembrane hidden Markov model (TMHMM).