Table II. List of mutants revealed by a genome-wide screen that are affected in MCC formation.

Name	ORF	Main biological process	Cellular compartment	Strength of phenotype
				HUP1	Can1	Sur7	Filipin
Level I
ERG2	YMR202W	Ergosterol biosynthesis	ERc	+++	+++	++	++
ERG24	YNL280C	Ergosterol biosynthesis	ERa	+++	+++	+	++
ERG6	YML008C	Ergosterol biosynthesis	ERa	+++	++	+	++
NCE102	YPR149W	Nonclassical protein secretion	Plasma membraned	+++	+++	++	+
OCH1	YGL038C	N-glycosylation	Golgia	+++	+++	+	+
PIL1	YGR086C	Endocytosis (eisosome)	Cytoplasm–plasma membrane associatedb	+++	+++	++	++
Level II
COG1	YGL223C	Intra-Golgi vesicle-mediated transport	Golgia	++	+++	+	−
GOS1	YHL031C	Intra-Golgi vesicle-mediated transport	Golgib	++	+	+	−
OPI3	YJR073C	Phosphatidylcholine biosynthesis	ERa	++	+++	+	−
RVS161	YCR009C	Polarization of the actin cytoskeleton, endocytosis, and cell polarity	Cytoplasm–plasma membrane associatedb	++	++	+	−
SUR4	YLR372W	Sphingolipid biosynthesis	ERa	+++	+++	+	−
TAF14	YPL129W	RNA polymerase II transcription initiation and chromatin modification	Nucleusa	+	+	+	−
VPS52	YDR484W	Retrograde transport and endosome to Golgi	Golgia	+++	++	+	−
VPS54	YDR027C	Retrograde transport and endosome to Golgi	Golgia	+++	+++	+	−
Level III
CAX4	YGR036C	N-glycosylation	ERa	+++	++	−	−
ELP6	YMR312W	Regulation of transcription	Nucleusc	+	+	−	−
ERG5	YMR015C	Ergosterol biosynthesis	ERa	+++	+++	−	−
FYV6	YNL133C	Unknown	Nucleusa	+++	+	−	−
HNT3	YOR154W	Unknown	Cytoplasma	+++	+++	−	−
MNN10	YDR245W	N-glycosylation	Golgia	++	−	−	−
MNN11	YER001W	N-glycosylation	Golgia	+	+	−	−
NOT3	YIL038C	Regulation of transcription	Cytoplasma	+	+	−	−
PEP12	YOR036W	Golgi to vacuole transport	Golgib	+++	+++	−	−
PEP7	YDR323C	Golgi to vacuole transport	Endosomea	+++	++	−	−
PER1	YCR044C	GPI anchor biosynthesis	ERa	++	++	−	−
RPL13B	YMR142C	Translation	Cytoplasma	++	++	−	−
SLM1	YIL105C	Actin cytoskeleton organization	Cytoplasm–plasma membrane associatedd	++	+	−	−
SWA2	YDR320C	Vesicular transport	ERb	++	+	−	−

GPI, glycosylphosphatidylinositol. The phenotypes are categorized as follows: wild type–like, −; weak, +; medium, ++; strong, +++. Examples are given in Fig. 2.

^aGFP localization database; University of California, San Fransisco.

^bInferred from direct assays.

^cInferred from site of activity.

^dThis study.