Figure 2.

Smc1 and Smc3 coimmunoprecipitate each other as well as themselves. 293T cells were transfected with the appropriate plasmids. After 48 h, cell lysates were prepared and used for IP. The loaded input samples were equivalent to 10% of IP samples. (A) Myc-Smc1 and Myc-Smc3 coimmunoprecipitated endogenous Smc3 and Smc1 as well as endogenous Smc1 and Smc3, respectively. (B and C) Cell lysates were treated with or without DNase I and RNase A before co-IP of Smc1–Smc1 (B) and Smc3–Smc3 (C) was performed. EV, empty vector.