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. 2008 Dec 15;183(6):1019–1031. doi: 10.1083/jcb.200801157

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© 2008 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Each cohesin complex contains only one copy of SA1 or SA2. 293T cells were cotransfected with the respective constructs. 48 h after transfection, IP was performed. SA1 and SA2 were detected using the corresponding antibodies shown on the blots. (A) HA and Flag epitopes were tagged to the NT of SA1 and SA2. (B and C) HA and Myc epitopes were tagged to the CT of SA1 and SA2. EV, empty vector; *, splicing SA1-Myc or SA2-Myc; **, nonspecific band. Black lines indicate that intervening lanes have been spliced out.