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. 2008 Dec 15;183(6):1019–1031. doi: 10.1083/jcb.200801157

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© 2008 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Rad21–Rad21 interaction determined by yeast two-hybrid assay. (A) Expression of Rad21 wild type (1–631 aa), CT-L (254–631 aa), and CT (451–631 aa) in yeast was analyzed with WBs using Rad21 CT–specific pAb. (B) A LacZ reporter assay was used to probe the Rad21–Rad21 interaction. Yeast cells cotransfected with pC97 and pC86 empty vector is shown as the negative control. (C) pC97 GAL4BD and pC86 GAL4AD were used as the positive control. Positive protein–protein interaction is shown in blue in th LacZ reporter assay.