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. 2008 Dec 15;183(6):1007–1017. doi: 10.1083/jcb.200807006

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© 2008 Reber et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Dynamic measurements of centrosomally nucleated microtubules in metaphase or anaphase. CSF-arrested egg extracts were supplemented with Cy3-tubulin and purified human centrosomes and were triggered to enter anaphase as indicated. (A) Microtubules were visualized by time-lapse microscopy in 2-s intervals, and microtubules were tracked manually. Still images (after 15 min) are shown. (B) Parameters of dynamic instability were determined from data gained by time-lapse imaging using a Matlab macro. MT, microtubules; vg, growth rate; vs, shrinkage rate; fcat, catastrophe frequency; fres, rescue frequency. Bar, 10 μm.