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. 2008 Dec 1;22(23):3242–3254. doi: 10.1101/gad.1738708

Figure 3.

Figure 3.

Expression of miR-133a and miR-1 in mutant mice. (A) Genotypes of offspring from miR-133a mutant intercrosses. Timed matings were set up from miR-133a-1neo/neo; miR-133a-2neo/+ intercrosses, or from miR-133a-1neo/+; miR-133a-2neo/neo intercrosses to obtain dKO embryos. dKO mice were also intercrossed with miR-133a-1neo/neo; miR-133a-2neo/+ mice to obtain dKO mice at P1 and P10. Mice were genotyped at the indicated ages. Numbers of total mice analyzed, dKO mice observed, and dKO mice expected, based on Mendelian inheritance, are shown. (B) Northern blot analysis of heart and skeletal muscle RNA from wild-type and mutant mice at P1. Ten micrograms of RNA from skeletal muscle and heart tissues were used in the Northern blots. 32P-labeled Star-Fire probes for miR-133a and miR-1 were used. U6 probe was used as a loading control. (C) Expression levels of miR-133a and miR-1 in hearts of wild-type and dKO mutant mice at P1 detected by real-time PCR. Expression of miR-133a and miR-1 was normalized to U6 (n = 3 for each genotype). Error bars indicate the SEM.