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. 1986 Jul;53(1):95–98. doi: 10.1128/iai.53.1.95-98.1986

Antibodies to bacterial vaccines demonstrating specificity for human choriogonadotropin (hCG) and immunochemical detection of hCG-like factor in subcellular bacterial fractions.

G J Domingue, H F Acevedo, J E Powell, V C Stevens
PMCID: PMC260080  PMID: 3721581

Abstract

Investigations were done to determine whether vaccines prepared with chemically killed Staphylococcus haemolyticus RU1 and Streptococcus bovis AV46 (bacteria that have been demonstrated to express human choriogonadotropin [hCG]-like material on their surface) elicited antibodies in rabbits with specificity for hCG determinants. In addition, the anatomical locus of the hCG-like factor was determined by separation of bacterial subcellular fractions. The results demonstrated that these bacterial vaccines elicited antibodies immunologically similar to those antibodies produced in response to the whole human trophoblastic hormone, a similarity extending even to cross-reactivity with human luteinizing hormone. The bacterial hCG-like material appeared to be localized in the membranes of the cell wall, and most was present in the soluble membranous and cytoplasmic constituents. Its expression in bacteria was a strain characteristic and not a species characteristic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acevedo H. F., Campbell-Acevedo E., Kloos W. E. Expression of human choriogonadotropin-like material in coagulase-negative Staphylococcus species. Infect Immun. 1985 Dec;50(3):860–868. doi: 10.1128/iai.50.3.860-868.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Acevedo H. F., Koide S. S., Slifkin M., Maruo T., Campbell-Acevedo E. A. Choriogonadotropin-like antigen in a strain of Streptococcus faecalis and a strain of Staphylococcus simulans: detection, identification, and characterization. Infect Immun. 1981 Jan;31(1):487–494. doi: 10.1128/iai.31.1.487-494.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Acevedo H. F., Slifkin M., Pouchet G. R., Pardo M. Immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients. Cancer. 1978 Apr;41(4):1217–1229. doi: 10.1002/1097-0142(197804)41:4<1217::aid-cncr2820410401>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  4. Asch R. H., Fernandez E. O., Magnasco L. A., Pauerstein C. J. Demonstration of a chorionic gonadotropin-like substance in rabbit morulae. Fertil Steril. 1978 Apr;29(4):444–446. doi: 10.1016/s0015-0282(16)43222-x. [DOI] [PubMed] [Google Scholar]
  5. Backus B. T., Affronti L. F. Tumor-associated bacteria capable of producing a human choriogonadotropin-like substance. Infect Immun. 1981 Jun;32(3):1211–1215. doi: 10.1128/iai.32.3.1211-1215.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Boorstein W. R., Vamvakopoulos N. C., Fiddes J. C. Human chorionic gonadotropin beta-subunit is encoded by at least eight genes arranged in tandem and inverted pairs. Nature. 1982 Dec 2;300(5891):419–422. doi: 10.1038/300419a0. [DOI] [PubMed] [Google Scholar]
  7. Chou J. Y. Regulation of the synthesis of human chorionic gonadotropin by strains of HeLa cells in culture. In Vitro. 1978 Sep;14(9):775–778. doi: 10.1007/BF02617971. [DOI] [PubMed] [Google Scholar]
  8. Cohen H., Strampp A. Bacterial synthesis of substance similar to human chorionic gonadotrophin. Proc Soc Exp Biol Med. 1976 Jul;152(3):408–410. doi: 10.3181/00379727-152-39407. [DOI] [PubMed] [Google Scholar]
  9. Domingue G. J., Schlegel J. U. Novel bacterial structures in human blood: cultural isolation. Infect Immun. 1977 Feb;15(2):621–627. doi: 10.1128/iai.15.2.621-627.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Domingue G., Johnson E. Isolation of subcellular fractions containing immunogenic enterobacterial common antigen. Z Immunitatsforsch Exp Klin Immunol. 1974 Nov;148(1):23–38. [PubMed] [Google Scholar]
  11. Fiddes J. C., Goodman H. M. The gene encoding the common alpha subunit of the four human glycoprotein hormones. J Mol Appl Genet. 1981;1(1):3–18. [PubMed] [Google Scholar]
  12. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kellen J. A., Kolin A., Acevedo H. F. Effects of antibodies to choriogonadotropin in malignant growth. I. Rat 3230 AC mammary adenocarcinoma. Cancer. 1982 Jun 1;49(11):2300–2304. doi: 10.1002/1097-0142(19820601)49:11<2300::aid-cncr2820491117>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  14. Kellen J. A., Kolin A., Mirakian A., Acevedo H. F. Effects of antibodies to choriogonadotropin in malignant growth. II. Solid transplantable rat tumors. Cancer Immunol Immunother. 1982;13(1):2–4. doi: 10.1007/BF00200193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maruo T., Cohen H., Segal S. J., Koide S. S. Production of choriogonadotropin-like factor by a microorganism. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6622–6626. doi: 10.1073/pnas.76.12.6622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McManus L. M., Naughton M. A., Martinez-Hernandez A. Human chorionic gonadotropin in human neoplastic cells. Cancer Res. 1976 Sep;36(9 Pt 2):3476–3481. [PubMed] [Google Scholar]
  17. Roth J., LeRoith D., Shiloach J., Rosenzweig J. L., Lesniak M. A., Havrankova J. The evolutionary origins of hormones, neurotransmitters, and other extracellular chemical messengers: implications for mammalian biology. N Engl J Med. 1982 Mar 4;306(9):523–527. doi: 10.1056/NEJM198203043060907. [DOI] [PubMed] [Google Scholar]
  18. Slifkin M., Pardo M., Pouchet-Melvin G. R., Acevedo H. F. Immuno-electron microscopic localization of a choriogonadotropin-like antigen in cancer-associated bacteria. Oncology. 1979;36(5):208–210. doi: 10.1159/000225343. [DOI] [PubMed] [Google Scholar]
  19. Stevens V. C., Powell J. E., Lee A. C., Griffin D. Antifertility effects of immunization of female baboons with C-terminal peptides of the beta-subunit of human chorionic gonadotropin. Fertil Steril. 1981 Jul;36(1):98–105. doi: 10.1016/s0015-0282(16)45627-x. [DOI] [PubMed] [Google Scholar]
  20. Tsong Y. Y., Chang C. C., Nash H. A. Formulation of a potential antipregnancy vaccine based on the beta-subunit of human chorionic gonadotropin (beta-hCG). I. Alternative macromolecular carriers. J Reprod Immunol. 1985 Feb;7(2):139–149. doi: 10.1016/0165-0378(85)90068-3. [DOI] [PubMed] [Google Scholar]
  21. Wilson T. S., McDowell E. M., McIntire K. R., Trump B. F. Elaboration of human chorionic gonadotropin by lung tumors: an immunocytochemical study. Arch Pathol Lab Med. 1981 Apr;105(4):169–173. [PubMed] [Google Scholar]

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