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. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: Biochim Biophys Acta. 2006 May 4;1764(7):1252–1259. doi: 10.1016/j.bbapap.2006.04.009

Fig. 4.

Fig. 4

EMSA on wt bZIP bound to AP-1, C/EBP, XRE1, Partial site, E-box, and HRE. Each lane contains ~3000 cpm 32P-endlabeled 24-mer duplex. Lanes 1–7: free DNA. Lanes 8–14: DNA in the presence of 500 nM wt bZIP. Lanes 1 and 8, AP-1; lanes 2 and 9, C/EBP; lanes 3 and 10, XRE1; lanes 4 and 11, E-box; lanes 5 and 12, HRE; lanes 6 and 13, Partial site; lanes 7 and 14, nonspecific control. EMSA was run at 120 V for 4 h. I indicates bandshift from dimeric wt bZIP complexation. II and III indicate wt bZIP aggregates that bind to the duplexes and cause bandshifts (see text for discussion).