. Author manuscript; available in PMC: 2008 Dec 11.

Published in final edited form as: Biochemistry. 2008 Jul 22;47(33):8786–8795. doi: 10.1021/bi8007824

Table 4.

Kinetic Parameters of Wild-Type and Mutant Factor VIII^a

factor VIII	K_m(app) (nM)	V_max for Xa generation (nM min⁻¹)
wild type	32 ± 5.7	276 ± 21
E720A	56 ± 7.8	308 ± 25
D721A	43 ± 9.7	257 ± 20
E724A	135 ± 16	277 ± 9.6
D725A	173 ± 8.9	259 ± 14
E720A/D721A	78 ± 13	269 ± 26
E724A/D725A	230 ± 25	248 ± 9.2

Kinetic parameters were determined from initial rate data using the Michaelis-Menten equation and nonlinear least squares regression. Experiments were performed at least three separate times, and average values are shown.