Table 1.

Expression ratio of mPGE synthase and hPGD synthase mRNA in lung tissue from different treatment groups^a

Expression ratio	Non-sensitized	Untreated HDM-sensitized	MM ASO HDM-sensitized	COX-2 ASO HDM-sensitized
mPGE synthase/GAPDH	1.105 ± 0.09	1.047 ± 0.09	1.068 ± 0.13	0.624 ± 0.13*
hPGD synthase/GAPDH	0.369 ± 0.06	0.417 ± 0.06	0.335 ± 0.05	0.365 ± 0.06

^a mRNA expression was analyzed by conventional reverse transcriptase-polymerase chain reaction and the expression ratios compared with GAPDH were determined by densitometry. No differences were observed in hPGD synthase mRNA expression, either between non-sensitized and sensitized animals or between HDM-sensitized animals treated with mismatched antisense oligonucleotides and animals treated with COX-2 antisense oligonucleotide. In contrast, mPGE synthase expression was reduced in the selective COX-2 oligonucleotide-treated mice compared with the other experimental groups. Data are shown as the mean ± SEM (*p< 0.05). ASO, antisense oligonucleotide; MM, mismatched oligonucleotide (n = 12).