Fig. 3.

Detection of human cytomegalovirus (HCMV) in primary glioblastoma multiforme (GBM) cultures. Short-term GBM cultures from two patients were stained with a variety of monoclonal antibodies to detect HCMV proteins or astrocyte markers (GFAP). Cells were fixed, permeabilized, and stained with equimolar concentrations of isotype control antibodies (mouse IgG), anti-GFAP antibody, anti-HIV p17, anti-HCMV glycoprotein B (gB), anti-CD45, and anti-HCMV pp28. Photos demonstrate strong nuclear reactivity of astrocytic tumor cells to anti-HCMV gB and pp28 with scant cytoplasmic staining in two primary GBM cultures. Isotype controls and HIV p17 staining were negative in all samples examined. CD45 immunoreactivity was observed in scattered hematopoietic cells in one culture (patient 1 GBM, bottom left panel), but did not stain astrocytic tumor cells.