Angular dependence of the wide-angle X-ray scattering intensity, I(ϕ), from aligned DPPC multilayers, with and without 40 mol% cholesterol, at 45 °C [144]. (Note: I(ϕ) for DPPC+Chol is offset from zero by +0.15.) Black lines are fits to a single distribution of chain orientations, based on the Maier-Saupe of potential strength m : I(ϕ)∝exp(½mcos2ϕ)×I0(½mcos2ϕ), where I0 is a modified Bessel function of the first kind. Inset: dependence on cholesterol content of the chain order parameters determined from the angular fitting of the X-ray intensities (m=11.3±0.4 and 3.0±0.01 for DPPC with and without Chol, respectively), or from 2H-NMR of the perdeuterated lipid chains [147].