Table 3. Effects and interactions of all tested parameters including signal-to-noise t-ratios for each case, based on number of peaks in the output spectra.

(A) Effects for each parameter were calculated using the average number of peaks found for each of the 16 configurations. Signal-to-noise-ratios were then found by dividing each effect by the calculated standard deviation. (B) Similarly, interactions and their respective t-ratios were also calculated by using the average number of peaks in accordance with the standard deviation.

A. Main effects
	Factors of Interest
	A	B	C	D
Main Effects	144.6	−38.79	5.042	−30.63
signal-to-noise t-ratio	9.993	−2.680	0.348	−2.116
significance (|te|>t95)	YES	YES	NO	YES

B. Interaction effects
	Interactions Between Factors
	AB	AC	AD	BC	BD	CD	ABC	ABD	BCD	ABCD
Interaction Effects	57.04	6.208	−44.29	4.292	19.46	4.458	6.958	29.96	−2.458	57.04
signal-to-noise t-ratio	3.941	0.429	−3.060	0.297	1.345	0.308	0.481	2.070	−0.170	0.705
significance (|te|>t95)	YES	NO	YES	NO	NO	NO	NO	YES	NO	NO