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. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: J Mol Biol. 2008 Mar 19;378(4):761–777. doi: 10.1016/j.jmb.2008.03.023

Figure 4.

Figure 4

Effects of Mg2+ on the chemical protection patterns of a blunt-ended DNA bound by RecBC. KMnO4 footprinting experiments of the RecBC-blunt-ended-DNA complex were performed in buffer M plus the indicated [NaCl] and [MgCl2] at 25°C as described in Materials and Methods. Lane 1 is a control with just the 5’-32P-labeled ssDNA top strand alone. The contents of lanes 2 through 9 are indicated in the figure. The asterisk in the inset shows the position of the 32P-label on the DNA. The reference DNA band is indicated by ref and the uncut sample DNA band is denoted by uncut.