Table 2.

Temperature dependence of the observed enthalpic change (ΔH_obs) for RecBC binding to one end of the DNA VI series molecules with n = 0 or 6. Observed heat capacity change (ΔC_p,obs) values are obtained from linear least-square analyses of the observed enthalpic change data. (buffer M plus 100 mM NaCl and the indicated [MgCl₂])

Temperature (°C)	DNA with twin (dT)6 tails		Blunt-ended DNA
	ΔHobs (kcal mol−1) in 10 mM MgCl2	ΔHobs (kcal mol−1) in 0 mM MgCl2	ΔHobs (kcal mol−1) in 10 mM MgCl2	ΔHobs (kcal mol−1) in 0 mM MgCl2
5	−32 ± 3	−31 ± 3	ND	ND
10	−38 ± 3	−35 ± 3	ND	ND
15	−48 ± 3	−47 ± 3	−3 ± 3	−7 ± 3
17	ND	ND	−5 ± 3	−8 ± 3
20	−56 ± 3	−53 ± 2	−8 ± 3	−9 ± 3
25	−64 ± 3	−61 ± 2	−15 ± 2	−11 ± 2

ss-(dT)n tail length (n)	ΔCp,obs (kcal mol−1 K−1) in 10 mM MgCl2	ΔCp,obs (kcal mol−1 K−1) in 0 mM MgCl2
0	−1.2 ± 0.2	−0.5 ± 0.3
6	−1.6 ± 0.3	−1.6 ± 0.4