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Temperature dependence of the equilibrium constants (KBC) for RecBC binding to a blunt-ended DNA. (buffer M plus 100 mM NaCl and the indicated [MgCl2])
| Temperature (°C) | KBC (107 M−1) in 10 mM MgCl2 | KBC (107 M−1) in 0 mM MgCl2 |
|---|---|---|
| 5 | 2.0 ± 0.7 | 0.8 ± 0.1 |
| 15 | 3.7 ± 0.7 | 0.8 ± 0.1 |
| 25 | 1.6 ± 0.3 | 0.56 ± 0.05 |
