TABLE 2.
Crosses to examine chromosome-specific LOH
| Conditional CEN | Diploid genotype | LOH phenotype | Gal-CEN parent | Test parent |
|---|---|---|---|---|
| 1 | ADE1/ade1 | Adenine auxotroph | DY6297/DY6281a | K393-27B/-11B |
| 2 | LYS2/lys2 | Lysine auxotroph | W3616-3C/-3A | K381-15C/-10A |
| 3 | MATa/α | Mating competent | DY6296/DY6280 | K381-15C/-10A |
| 3 | HIS4/his4 | Histidine auxotroph | DY3930b | WY387 |
| 3 | THR4/thr4 | Threonine auxotroph | DY3930b | WY387 |
| 4 | CDC7/cdc7-4 | No growth at 37° | DY6298 | H7C4A1 |
| 4 | MBP1/mbp1 | CTS1-lacZ expression | DY4512b | WY352 |
| 4 | TRP1/trp1 | Tryptophan auxotroph | DY4512b | WY352 |
| 4 | TRP4/trp4 | Tryptophan auxotroph | DY4512b | DY6715 |
| 4 | CDC9/cdc9 | No growth at 37° | DY2877b | DY6715 |
| 4 | ARO1/aro1B | Tyrosine auxotroph | DY2877b | DY6715 |
| 4 | swi5∷TRP1/SWI5 | Tryptophan auxotroph | DY2877b | WY352 |
| 4 | ho-lacZ/ho | Loss of lacZ expression | DY2877b | DY6715 |
| 4 | ADE8/ade8 | Adenine auxotroph | DY2877b | DY6715 |
| 5 | HIS1/his1 | Histidine auxotroph | DY6299/DY6283 | K393-27B/-11B |
| 6 | HIS2/his2 | Histidine auxotroph | DY6300/DY6284 | K393-27C/-2D |
| 7 | ADE6/ade6 | Adenine auxotroph | DY6301/DY6285 | K381-15C/-10A |
| 7 | TRP5/trp5 | Tryptophan auxotroph | DY6301/DY6285 | K393-27B/-11B |
| 8 | ARG4/arg4 | Arginine auxotroph | DY6302/DY6286 | K381-15C/-10A |
| 9 | LYS1/lys1-1 | Lysine auxotroph | DY6303/DY6287 | K393-27C/-2D |
| 10 | MET3/met3 | Methionine auxotroph | DY6304/DY6288 | K393-27B/-11B |
| 11 | MET14/met14 | Methionine auxotroph | DY6305/DY6289 | K381-15C/-10A |
| 12 | ASP5/asp5 | Aspartate auxotroph | DY6306/DY6290 | K381-15C/-10A |
| 13 | LYS7/lys7 | Lysine auxotroph | DY6307/DY6291 | K393-27B/-11B |
| 14 | MET4/met4 | Methionine auxotroph | W3617-1B/-1A | K393-27C/-2D |
| 14 | PET8/pet8 | No growth on glycerol | W3617-1B/-1A | K393-27C/-2D |
| 15 | PET17/pet17 | No growth on glycerol | DY6309/DY6293 | K381-15C/-10A |
| 16 | ARO7/aro7-1 | Tyrosine auxotroph | DY6310/DY6294 | K381-15C/-10A |
a
Strains separated by slashes indicate congenic MATa and MATα strains.
b
Strains were transformed to integrate Gal-CEN insertion.