TABLE 2.
NHEJ phenotype of Lif1 C-terminal mutants
|
YKU80
|
yku80Δ605–629
|
|||
|---|---|---|---|---|
| LIF1 | Total | HO(+2) | Total | HO(+2) |
| wt | 1a | 1 | 2 | 8 |
| Δ∷kanMX4 | 16 | >1000 | 20 | ∞ |
| Δ410-421 | 1.6 | 1.5 | 16 | 68 |
| T387A | 1 | 1.2 | 4 | 14 |
| S383A, S385A, T387A | 1 | 1.4 | 4 | 13 |
| T413A | 1.6 | 5 | 1 | 10 |
| S415A | 1 | 1 | 4 | 16 |
| T417A | 1.4 | 1.7 | 14 | 79 |
| T417E | NDb | 1.4 | 10 | 74 |
| T417D | 1 | 1 | 8 | 58 |
| T421A | 1 | 1 | 3 | 8 |
| T413A, S415A, T417A, T421 | 1.5 | 1.6 | 19 | 100 |
| T387A, T417A | 2 | 1 | 20 | >1000 |
wt, wild type.
a
Numbers indicate the average fold decrease as compared to YKU80 XRS2 yeast for total and HO(+2) NHEJ in the suicide deletion assay.
b
Not determined.