TABLE 3.
Karyotype aberrations induced by dicentric chromosome formation
| Metaphase nuclei
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Abnormal karyotypes (%)
|
Normal karyotype (%) | ||||||||
| Genotype | Time (hr) | Nuclei, brains (n) | 0 | 1 | 2 | 2+ | 4N | ||
| A. | Dc2(FrTr1B) | No HS | 154, 3 | 0.65 | 0 | 1.3 | 0 | 98 | 0 |
| B. | Dc2(FrTr1B) | +24 | 167, 3 | 16 | 7.8 | 48 | 6.6 | 22 | 1 |
| C. | Dc2(FrTr1B) | +48 | 82, 4 | 26 | 3.6 | 19 | 5 | 46 | 0 |
| D. | Dc2(FrTr1B) | +96 | 59, 2 | 0 | 0 | 3.4 | 6.8 | 90 | 0 |
| E. | Dc2(FrTr1B); p53 | No HS | 154, 3 | 1 | 0 | 0 | 1 | 98 | 0 |
| F. | Dc2(FrTr1B); p53 | +24 | 207, 3 | 20 | 2 | 55 | 10 | 13 | 0 |
| G. | Dc2(FrTr1B); p53 | +48 | 211, 4 | 20 | 3 | 52 | 10 | 15 | 6 |
| H. | Dc2(FrTr1B); p53 | +96 | 115, 3 | 4 | 1.7 | 59 | 7.8 | 28 | 1 |
| I. | DcX(105) | No HS | 97, 3 | 0 | 1 | 1 | 0 | 98 | 0 |
| J. | DcX(105) | +24 | 105, 4 | 9 | 3 | 72 | 3 | 12 | 1 |
| K. | DcX(105) | +48 | 223, 4 | 9 | 1 | 55 | 8 | 26 | 1 |
| L. | DcX(105) | +96 | 179, 4 | 4 | 1 | 11 | 4 | 80 | 1 |
| M. | DcX(105); p53 | No HS | 117, 3 | 0 | 0 | 1 | 0 | 99 | 0 |
| N. | DcX(105); p53 | +24 | 247, 3 | 1 | 2 | 74 | 9 | 8 | 6 |
| O. | DcX(105); p53 | +48 | 491, 4 | 9 | 1 | 68 | 10 | 12 | 11 |
| P. | DcX(105); p53 | +96 | 303, 4 | 10 | 2 | 51 | 10 | 27 | 5 |
| Q. | DcX(105); lok | No HS | 224, 3 | 1 | 1 | 1 | 0 | 96 | 0 |
| R. | DcX(105); lok | +24 hr | 375, 4 | 10 | 2 | 71 | 10 | 7 | 0 |
| S. | DcX(105); lok | +48 | 172, 3 | 5 | 1 | 59 | 13 | 21 | 3 |
| T. | DcX(105); lok | +96 | 268, 3 | 2 | 4 | 39 | 18 | 37 | 3 |
| U. | Dc3(FrTr)1D | No HS | 112, 3 | 0 | 0 | 1 | 0 | 98 | 0 |
| V. | Dc3(FrTr)1D | +24 | 187, 3 | 7 | 10 | 57 | 1 | 21 | 2 |
| X. | Dc3(FrTr)1D | +48 | 146, 3 | 7 | 7 | 41 | 4 | 44 | 1 |
| Y. | Dc3(FrTr)1D | +96 | 193, 3 | 3 | 0.5 | 7 | 0.5 | 89 | 1 |
| Z. | lok; Dc3(FrTr)1D | No HS | 257, 3 | 0 | 0 | 2 | 0.4 | 98 | 0 |
| AA. | lok; Dc3(FrTr)1D | +24 | 376, 3 | 13 | 5 | 58 | 8 | 17 | 0 |
| BB. | lok; Dc3(FrTr)1D | +48 | 308, 3 | 24 | 3 | 46 | 3 | 23 | 8 |
| CC. | lok; Dc3(FrTr)1D | +96 | 201, 4 | 14 | 1 | 38 | 6 | 40 | 0 |
| DD. | mei-41; lok; Dc3(FrTr)1D | No HS | 111, 2 | 0 | 0 | 2 | 0 | 98 | 0 |
| EE. | mei-41; lok; Dc3(FrTr)1D | +24 | 266, 3 | 9 | 6 | 67 | 5 | 13 | 5 |
| FF. | mei-41; lok; Dc3(FrTr)1D | +48 | 313, 3 | 20 | 2 | 48 | 4 | 25 | 6 |
Abnormal karyotypes were scored for the presence and number of acentric fragments. Time indicates time after heat-shock induction of P{70FLP}3F, except genotypes that included mei-41, which used P{70FLP}4A. The number of brains and number of metaphase nuclei scored are given. 4N indicates the number of tetraploid nuclei. HS, heat shock. Genotypes are as indicated in Table 1.