Figure 7.—

Effects of TSA treatment on the accumulation of hairpin RNA and siRNA. (A) Time course of hairpin RNA accumulation after TSA treatment. Samples were isolated at various time points after TSA inoculation and Northern blot analyses were carried out using a DIG-labeled sense-aadA RNA probe. Detection of the RbcS2 mRNA was also carried out to confirm equal loading of RNA. (B) Time course of siRNA accumulation after TSA treatment. A DIG-labeled sense-aadA RNA probe was used to detect the siRNA from small-sized RNA fractions isolated at different time points after TSA treatment. Ethidium bromide staining of RNA was used to confirm equal loading. +180: cells were first inoculated in TSA–TAP medium for 180 min, followed by a recovery period of 180 min in fresh TAP medium.