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The sir2-276 mutation confers a temperature-sensitive mating defect. The indicated strains were transformed with a plasmid expressing SIR2, a vector or a plasmid expressing sir2-276 (pCLW18, pRS316, and pCLW19 for the MATα sir2 strain, RS1717; pCLW20, pRS313, and pCLW21 for the MATa sir2 strain, RS869). Mating was measured at 25° or 37° as indicated.
