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The Sir2-276 mutant protein is stable at 37°. A Western with an antibody to Sir2 showed that similar amounts of Sir2 were present in wild-type cells and the sir2-276 mutant grown at 25° or at 37°. No signal was seen for a sir2Δ strain. An antibody to tubulin was used to show that similar amounts of total protein were present in all lanes.
