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nfi1Δ strains display a moderate increase in the angle at which the spindle aligns along the mother-bud axis. (A) The angle of alignment (α). (B) Preanaphase cells displaying short bipolar spindles labeled with GFP-Tub1p (pRM4419) were analyzed in strains deleted for SIZ1 (yRM4769), NFI1 (yRM4755), kar9Δ (yRM6172), and wild type (yRM1756 or yRM1757). Each strain was counted on at least 4 separate days.
