TABLE 4.
Sumoylation mutants do not display synthetic lethality with either KAR9 or dynein pathway mutants
| Cross | Viability | Tetrad (PD:TT:NPD) | Double mutant (viable/predicted) |
|---|---|---|---|
| cst9Δ × jnm1Δ | Viable | 25 (3:16:6) | 27/28 |
| siz1Δ × jnm1Δ | Viable | 24 (3:19:2) | 22/23 |
| cst9Δ × dhc1Δ | Viable | 27 (3:20:4) | 28/28 |
| siz1Δ × dhc1Δ | Viable | 22 (4:14:4) | 22/22 |
| nfi1Δ × dhc1Δ | Viable | 27 (2:21:4) | 28/29 |
| SMT3 × jnm1Δ | Viable | 27 (8:15:4) | 20/23 |
| smt3-11 × jnm1Δ | Viable | 28 (6:16:6) | 28/28 |
| smt3-12 × jnm1Δ | Viable | 16 (2:12:2) | 16/16 |
| cst9Δ × kar9Δ | Viable | 31 (7:18:6) | 28/30 |
| siz1Δ × kar9Δ | Viable | 28 (2:21:5) | 30/31 |
| nfi1Δ × kar9Δ | Viable | 30 (9:19:2) | 23/23 |
| SMT3 × kar9Δ | Viable | 33 (5:25:3) | 30/31 |
| smt3-11 × kar9Δ | Viable | 19 (1:14:4) | 20/22 |
| smt3-12 × kar9Δ | Viable | 20 (3:11:6) | 23/23 |
Double mutants between sumoylation mutants and KAR9 or dynein pathway mutants were generated by meiotic crosses and scored for growth after 2–3 days. The tetrads were incubated at 30° except SMT3, smt3-11, and smt3-12 crosses, which were grown at 23°. The double mutant, single mutant, and wild type exhibited no difference in growth at 30° or 23°. Thus the double mutants were scored as viable. The following parental strains were used in the crosses: cst9Δ (yRM7166), siz1Δ (yRM7140), nfi1Δ/siz2Δ (yRM7248), SMT3 (yRM6491), smt3-11 (yRM6493), smt3-12 (yRM6492), jnm1Δ (yRM6727), dhc1Δ (yRM373), and kar9Δ (yRM1340).