TABLE 1.

Comparison of the technical properties of the HRAII and the prototype Zeiss cSLO

Characteristic	HRAII	Zeiss cSLO
Laser type	488 nm argon	488 nm argon
Barrier filter	500 nm	521 nm
Laser output	275 μm	320 μm
Detector type	Photodiode	Photomultiplier tube
Field of view	15°/20°/30°	20°/40°
Confocal aperture	400 μm	2mm
Image dimensions	1536 × 1536 px/768 × 768 px	768 × 576 px
Nominal micrometers per pixel	1.15 (Rat)	3.07 (Rat)
	0.61 (Mouse)	1.61 (Mouse)
Exposure time per frame	0.125 s	0.04 s
Frame rate	8 frames/s	25 frames/s
Total exposure time for acquired sequence	32 s	10 s

px, pixels.