Table 3.
Notch, βcatenin and SoxE proteins induce sox10 transcript expression
| % positive ectopic sox10 expression | ||
| without heatshock | with heatshock | |
| Uninjected | ND | 1 (n = 91) |
| NotchΔEmv | 27(n = 33) | 54 (n = 41) |
| βcateninΔ | 27 (n = 86) | 54 (n = 95) |
| Sox9b | 5 (n = 41) | 44 (n = 34) |
Zebrafish embryos injected with heatshock plasmid constructs at the 1-cell stage were incubated for 6 hours before a 60 min 37°C heatshock. After a further 1 hour incubation at 28.5°C embryos were fixed and processed for ectopic sox10 expression by in situ hybridisation.