Table 1.

Oligonucleotide primers used in this study

Primer name	Sequence 5'-3'
Tc-5'-PmlNco	CACGTGCCATGGTTCTCATGTTTGACAGCTTATC
Tc-3'-PmlNsi	CACGTGAT GCATGGACTTCCGCGTTTCCAG
SpR-5'-HpaBspNhe	ATCGTTAACTCATGAGCTAGCCGTTCGTGAATACATGTTATAATA
SpR-3'-Hpa	ATCGTTAACTTATTTGCCGACTACCTTGGTGATCT
GFP-mu-5'-Bam	ATCGGATCCAAAACAATGGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCC
GFP-mu-3'-NotAvrBgl	GATATCGCGGCCGCCCTAGGTAGATCTTCCACCTCCACCTTTGTATAGTTCATCCATGCCATGTGTA
ABI3-5'-CDS-Rescue-3'-Test	CTCCTCCATGGTCATCTAACC
ABI3-3'-CDS-Rescue-5'-Test	GTACCCGAGTGTGCCACTTC
PAP85-Prom-Rescue-3'-Test	ACTTCCTAGGGTTGCTACTG
PAP85-Term-Rescue-5'-Test	ACCGGATGGTTTGGT TATGG
ABI3-L1-Rescue-5'-CDS1	agatctccggcgttggccgccacatgcaagcttttcatcgttgaagtggaCAAATACGCATACTGTTATCTGGCT
ABI3-L2-Rescue-3'-CDS1	tattacatatgtggctcatttcttgagtcgagcttacatatcgtttataaAACCTGATGTTCTGGGGAATATAG
ENTRY-L1	TTAGTTAGTTACTTAAGCTCGG
ENTRY-L2	TGAGACACGGGCCAGAGC
PAP85-Rescue-5'-Prom2	ttcctaccaccatgaccaaacttggaatcccgaaactccctcttcttgttTTGTCGTTTCCCGCCTTCAG
PAP85-Rescue-3'-Term2	atagctcgtattttttagcttatgtctaaaatacccctgagatcatgtggAACGTTCTCGAGGGGATCTTCTG
pCB-RB	CTGTGGTTGGCATGCACATA
tCUP2-Out	CGAGGTTAGACCTTCAGGAAATA
PAP85-Integ-Dest-5'3	acccattattgtcctcaaaaacaaacacatcaacaaaacaacaagaaaaaGATATCACAAGTTTGTACAAAAAAGC
PAP85-Integ-Dest-3'3	ttaaactcttaaactctttcctttcttaggactgtaaaaaagcattttcaGATATCACCACTTTGTACAAGAAAGC
PAP85-Dest-Integ-5'-Test	TGATGCGTACGAACACTC
Cm-5'-AS	ACGGTGGTATATCCAGTGATT
PAP85-Dest-Integ-3'-Test	GTTACATGCAGAGCG TTACC
C1	TTATACGCAAGGCGACAAGG
PAP85-S1	GCAGGGATCCT TGTTCCTTC

¹Upper-case letters correspond to unique recognition sequences in pJM1 and lower-case letters correspond to 50 bp regions homologous to ABI3.

²Upper-case letters correspond to unique recognition sequences in pWY109 and pWY107 and lower-case letters correspond to 50 bp regions homologous to PAP85.

³Upper-case letters correspond to unique recognition sequences in the RfA-Destination cassette of pMW138-1 and lower-case letters correspond to 50 bp regions homologous to PAP85.