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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Steroids. 2007 Dec 27;73(9-10):1008–1012. doi: 10.1016/j.steroids.2007.12.016

Figure 2. Effects of rT3 on microfilament organization in astrocytes.

Figure 2

Astrocytes were grown on p-lysine coated coverslips for 48 h in DMEM containing 10% calf serum and antibiotics. Twenty-four hours before staining, growth medium was replaced with serum-free DMEM containing 1 mg/ml BSA ± 10 nM rT3. After 24 hrs, coverslips were then washed, fixed with 4% paraformylaldehyde and the actin cytoskeleton visualized with alexafluor488-phallacidin as described in ref (41).