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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: Brain Res. 2008 Jun 13;1227:1–11. doi: 10.1016/j.brainres.2008.05.086

Figure 6. BDNF increases internalization of CXCR4.

Figure 6

SY5YTB8 cells (Kim et al., 1999) were incubated with medium control (0.1% BSA) or medium containing BDNF (10 ng/ml) for 5 or 30 min or 3 hr. Cells were then washed and treated with 2 mM NHS-LC-biotin for 30 min at 4°C to biotinylate cell surface proteins. Biotinylated proteins were precipitated with immobilized streptavidin. Precipitates were then electrophoresed on SDS-polyacrylamide gels and subjected to CXCR4 immunoblotting. A. Example of immunoblot. B. Immunoreactive bands were scanned and quantified by Quantity One 1-D Analysis Software. Data, expressed as intensity of immunoreactive bands, are the mean ± SEM of three independent experiments. *p<0.05 vs control.