Figure 1. Ion Chromatogram of Cys, CySS, CySSG, GSH, And GSSG Standard Mixture.
A standard containing S-carboxymethyl-cysteine (CM-Cys), cystine (CySS), cysteine-glutathione disulfide (CySSG), S-carboxymethyl-glutathione (CM-GSH) and glutathione disulfide (GSSG) was used to test chromatographic and mass spectrometric conditions, and the resulting ion chromatogram was viewed with m/z windows of 180.0307–180.0343 for CM-Cys, 241.0287–241.0335 for CySS, 427.0920–427.0960 for CySSG, 366.0929–366.1002 for CM-GSH, 613.1530-613.165 + 307.0801-307.0863 for singly and doubly charged GSSG, and the total ion chromatogram (TIC).
