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. Author manuscript; available in PMC: 2008 Dec 12.
Published in final edited form as: Circulation. 2008 Jun 9;117(24):3070–3078. doi: 10.1161/CIRCULATIONAHA.107.763870

Figure 2.

Figure 2

Proteotoxic stress in load-induced heart failure. A, Representative immunohistochemical staining revealing accumulation of ubiquitin-conjugated proteins in basal interventricular septum 72 hours after sTAB (scale bar=200 μm). B, Representative immunoblot probed for ubiquitin demonstrating induction of high-molecular-weight, ubiquitinated protein in pressure-stressed LV. C, Representative immunoblot of LV lysates 72 hours after sTAB or sham operation and probed for multiple protein chaperones, including CryAB, HSP25, BiP, and GADD45. D, Proteasome activity assayed 1 week after sTAB is increased (1.35±0.2; n=4) relative to sham-operated controls (1.0±0.3; n=4). RV indicates right ventricle; AV, aortic valve; and BS, basal septum. *P<0.05.