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. Author manuscript; available in PMC: 2008 Dec 12.
Published in final edited form as: Circulation. 2008 Jun 9;117(24):3070–3078. doi: 10.1161/CIRCULATIONAHA.107.763870

Figure 7.

Figure 7

Aggresomes are a proximal trigger of cardiomyocyte autophagy. A, NRVMs were transfected with GFP-tagged LC3 and subjected to proteasome inhibition (16 hours), resulting in redistribution of LC3 in an autophagosome-specific punctate pattern. Images of representative cells are depicted (scale bar=10 μm). B, Quantification of the numbers of cells manifesting autophagic activity (*P<0.05 relative to control). More than 300 cells were evaluated in each treatment condition in 2 independent experiments. C, Cells treated with 3-MA for 7 days were assayed for proteasome activity, revealing a robust increase in activity. *P<0.05.