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A) Localization of DsRed-PDE5a is at the z-band of the cardiac myocyte, similar to results reported using anti-sera, while it became diffuse in eNOS-/- cardiomyocytes. B) Over-expression of DsRed-PDE5a in neonatal myocytes increases enzyme activity. C) Enhanced PDE5a expression augments PE-induced stimulation of ANP and BNP gene expression.
