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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Immunity. 2008 May 29;28(6):799–809. doi: 10.1016/j.immuni.2008.03.019

Figure 3. Colocalization of TLR9 to early endosome markers in resting cells.

Figure 3

(A) Mouse splenic B cells were stained with antibodies specific for EEA1 labeled with Alexa 488 or for TLR9 labeled with Alexa 647. Shown are the confocal images of Alexa 488 and Alexa 647 merged with DIC images. (B) Mouse splenic B cells were stained with antibodies specific for transferrin receptor (TfR) labeled with Alexa 555 or for TLR9 labeled with Alexa 647. Shown are the confocal images of Alexa 555 and Alexa 488 merged with DIC images. Approximately 200 cells were analyzed from 3 independent experiments and a representative image is given.