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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7107–7111. doi: 10.4049/jimmunol.180.11.7107

FIGURE 4.

FIGURE 4

NF-κB and MAPK activation in NEMO-KR macrophages. A, Bone marrow macrophages were stimulated with LPS (100 ng/ml for 10 min). Whole cell lysates were boiled in 1% SDS and diluted to 0.1% SDS before NEMO immunoprecipitation (IP). Western blots prepared from NEMO immunocomplexes were probed with anti-Ub Abs (top). A portion of each lysate (5%) was directly blotted (IB) and probed with Abs for NEMO (loading control; middle) or phospho-IκBα (IκBα-P) (activation control; bottom). B and C, Peritoneal macrophages (5 × 106 cells/ml) were stimulated with LPS (100 ng/ml) for the indicated times. Whole cell lysates were analyzed on immunoblots using Abs specific for IκB proteins (B) or phosphorylated (p-) forms of MAPKs (C). Relative levels of β-actin and total Erk provide loading controls.