Figure 1.

Experimental timeline. Chloralose-anesthetized animals underwent 10 minutes of cardiac arrest followed by 2 hours of reperfusion under hyperoxic (100% O2) or normoxic (21% to 30% O2) conditions for the first hour. [1-13C]glucose (0.2 mg/kg) was infused from 30 to 60 minutes of reperfusion. During the second hour of reperfusion, the ventilator settings were adjusted to maintain arterial pO2 at >80 and <120 mm Hg. At the end of the second hour, brains were removed and immediately immersed in liquid N2 (see Methods for details).