TABLE 2. Hippocampal Amino Acid Concentrations (nmol/mg protein) After Cardiac Arrest and 2-Hour Reperfusion.
Nonischemic | Hyperoxic | Normoxic | |
---|---|---|---|
Aspartate | 111.9±18.3 | 101.1±13.2 | 96.2±24.0 |
Glutamate | 670.9±90.3 | 785.2±86.4 | 683.3±73.6 |
Glutamine | 171.8±70.7 | 334.2±73.9 | 235.7±67.5 |
GABA | 75.2±13.0 | 127.9±21.3 | 117.1±10.9 |
Alanine | 38.5±6.7 | 84.4±16.9* | 77.1±7.5 |
Amino acids were determined by HPLC on extracts of frozen hippocampi from sham-operated, nonischemic animals and from animals after 10 minutes of cardiac arrest and 2-hour reperfusion using hyperoxic or normoxic resuscitation (Methods). Values are mean±SE for n=7 nonischemic, 7 hyperoxic, and 6 normoxic animals.
Significantly different from nonischemic controls (P<0.05).