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. Author manuscript; available in PMC: 2009 Aug 22.
Published in final edited form as: Cell. 2008 Aug 22;134(4):624–633. doi: 10.1016/j.cell.2008.06.027

Figure 1. A role for Gle1 in translation.

Figure 1

(A) Cultures of gle1-2, gle1-4, rat7-1 (nup159), nup42Δ, gfd1Δ, and wild-type (WT) strains were spotted in five-fold serial dilutions on YPD alone or YPD containing 0.1 μg/ml cycloheximide or 0.4 mg/ml paromomycin, then incubated 2 days at 23°C.

(B) Cultures of gle1-4 and wild-type strains transformed with empty URA3/CEN plasmid (pURA3) or a GLE1/URA3/CEN (pGLE1) plasmid were spotted as in (A) on Ura− selective media with or without cycloheximide.

(C) Lysates of wild-type strains grown at 23°C were subjected to sucrose density fractionation and immunoblotted with α-Gle1 and α-Rps6 (ribosomal protein control). Ribosome distribution was determined by OD254.