Table I.
DC subsets in the LRLNs
| Treatmentb |
L-Selectinc |
P1d | P2e Naive vs. Treatment
|
||||
|---|---|---|---|---|---|---|---|
| DC Subset a | Ad2/β Gal-2 | LPS | −/− | +/+ | −/− vs. +/+ | −/− | +/+ |
| Lymphoid | – | – | 72.4 ± 3.1 | 77.8 ± 2.6 | NS | - | - |
| “ | + | – | 76.8 ± 1.9 | 81.2 ± 2.1 | NS | NS | NS |
| “ | + | – | 75.8 ± 2.7 | 78.0 ± 1.1 | NS | NS | NS |
| Plasmacytoid | – | – | 10.8 ± 2.3 | 11.8 ± 1.8 | NS | - | - |
| “ | + | – | 17.1 ± 1.2 | 14.6 ± 1.5 | NS | 0.038 | NS |
| “ | + | + | 15.3 ± 1.7 | 17.6± 2.3 | NS | NS | NS |
| Myeloid | – | – | 26.8 ± 3.0 | 21.5 ± 2.5 | NS | - | - |
| “ | + | – | 21.9 ± 2.0 | 17.2 ± 2.0 | NS | NS | NS |
| “ | + | + | 23.6 ± 1.0 | 21.1 ± 1.1 | NS | NS | NS |
a
Pulmonary DCs were gated on lymphocytes that were CD11clow TCRα β−; no CD11chigh cDCs were detected in the LNs.
b
DCs were derived from naive, Ad2/βGal-2-dosed, or Ad2/βGal-2 + LPS-dosed mice.
c
−/− = L-Selectin-deficient mice; +/+ = B6 mice. Data presented as mean ± SEM.
d
p-value between L-Sel−/− and L-Sel+/+ DC subsets (n = 10 mice/group); NS = not significant.
e
p-value between treatments within L-Sel−/− or L-Sel+/+ groups.