Table III.
DC subsets in the lungs
| Treatmentb |
L-Selectinc |
P1d | P2e Naive vs. Treatment
|
||||
|---|---|---|---|---|---|---|---|
| DC Subset a | Ad2/β Gal-2 | LPS | −/− | +/+ | −/− vs. +/+ | −/− | +/+ |
| Lymphoid | – | – | 57.6 ± 2.0 | 54.4 ± 4.4 | NS | - | - |
| “ | + | – | 44.5 ± 3.7 | 33.9 ± 2.7 | 0.035 | 0.007 | <0.001 |
| “ | + | – | 53.5 ± 2.9 | 38.6 ± 2.3 | <0.001 | NS | 0.004 |
| Plasmacytoid | – | – | 10.2 ± 0.7 | 14.2 ± 0.8 | 0.001 | - | - |
| “ | + | – | 17.3 ± 1.5 | 17.1 ± 1.6 | NS | <0.001 | NS |
| “ | + | + | 11.9 ± 1.4 | 14.5 ± 1.5 | NS | NS | NS |
| Myeloid | – | – | 11.4 ± 0.9 | 14.3 ± 2.0 | NS | - | - |
| “ | + | – | 17.5 ± 2.9 | 17.6 ± 3.8 | NS | NS | NS |
| “ | + | + | 17.8 ± 1.3 | 23.4 ± 1.3 | 0.006 | <0.001 | <0.001 |
| Conventional | – | – | 17.8 ± 1.0 | 24.0 ± 2.4 | 0.025 | - | - |
| “ | + | – | 33.8 ± 3.6 | 46.3 ± 3.9 | 0.029 | <0.001 | <0.001 |
| “ | + | + | 19.5 ± 2.5 | 31.8 ± 3.0 | 0.004 | NS | NS |
a
Pulmonary DCs were gated on lymphocytes that were CD11c TCRα β.
b
DCs were derived from naive, Ad2/βGal-2-dosed, or Ad2/βGal-2 + LPS-dosed mice.
c
−/− = L-Selectin-deficient mice; +/+ = B6 mice. Data presented as mean ± SEM.
d
p-value between L-Sel−/− and L-Sel+/+ DC subsets (n = 10 mice/group); NS = not significant.
e
p-value between treatments within L-Sel−/− or L-Sel+/+ groups.