. Author manuscript; available in PMC: 2009 Jul 15.

Published in final edited form as: J Immunol. 2008 Jul 15;181(2):1345–1356. doi: 10.4049/jimmunol.181.2.1345

Table IV.

Transgenic OT-1 CD8⁺ T cells remain unresponsive in Ad5-OVA-dosed L-Sel^−/− lungs

Experiment 1
	OT-1 → Recipient Tissue^a	Day Tested^b	% pentamer⁺ CD8⁺ T cells^b	P-Value^c	Effector: Target^d	% Lysis^d	P-Value^e
	L-Sel^+/+ lungs	2	24.5 ± 10.1	NS	25:1	15.7 ± 4.3	0.005
					50:1	36.9 ± 2.3	< 0.05
	L-Sel^−/− lungs	2	21.1 ± 2.7		25.1	−1.1 ± 2.6
					50:1	8.8 ± 2.9
	L-Sel ^+/+ lungs	3	15.2 ± 4.1	NS	25:1	38.0 ± 8.3	< 0.05
					50:1	66.1 ± 10.2	0.005
	L-Sel^−/− lungs	3	13.0 ± 0.3		25:1	6.5 ± 1.8
					50:1	8.5 ± 1.6

Experiment 2
	L-Sel^+/+ lungs	2	8.5 ±0. 9	NS	25:1	11.8 ±3.7	NS
					50:1	20.0 ± 4.3	NS
	L-Sel^−/− lungs	2	9.0 ±2.1		25:1	5.5 ± 3.2
					50:1	11.8 ±4.0
	L-Sel ^+/+ lungs	3	4.6 ± 0.4	0.040	25:1	17.0 ±3.8	0.007
					50:1	24.0 ± 1.4	<0.001
	L-Sel^−/− lungs	3	8.4 ±1.2		25:1	2.1 ±3.4
					50:1	5.9 ±3.3

Transgenic OT-1 T cells were purified to > 95% CD8⁺ T cells, and 5 x 10⁶ cells were injected i.v. into L-Sel^−/− or L-Sel^+/+ mice and challenged 24 h later with 1 x 10⁹ Ad5-OVA. Recipient lungs were harvested 2 or 3 days after challenge.

Total lymphocytes were harvested from lungs and stained with OVA_257–264 peptide in soluble MHC class I pentamer and with anti-CD8 mAb. Percent pentamer⁺ CD8⁺ T cells ±SEM were determined by flow cytometry.

P-value between the percentage of pentamer⁺ CD8⁺ T cells in L-Sel^+/+ and L-Sel^−/− lungs.

CTL experiments were performed using freshly isolated L-Sel^+/+ or L-Sel^−/− lymphocytes as effector cells measuring their lytic activity against E.G7-OVA target cells (percent lysis) at the indicated effector to target cell ratios.

P-value for OVA-specific cytolysis between L-Sel^+/+ and L-Sel^−/− lung lymphocytes.