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. 1986 Dec;54(3):723–727. doi: 10.1128/iai.54.3.723-727.1986

Eosinophil chemotactic lymphokine produced by egg-associated granulomas in murine schistosomiasis japonicum.

M Owhashi, H Maruyama, Y Nawa
PMCID: PMC260229  PMID: 3096889

Abstract

Eosinophil chemotactic factor (ECF) was detected in the culture supernatant of isolated intact egg granulomas from the livers of Schistosoma japonicum-infected mice. This factor had an apparent molecular weight of 15,000 by high-pressure liquid chromatography with an SW3000 column and bound to concanavalin A-Sepharose 4B. When cells obtained by enzymatic digestion of isolated granulomas were cultured under the presence of soluble egg antigen of S. japonicum or concanavalin A, ECF was also detected in the conditioned medium. The physicochemical nature of the ECF produced by concanavalin A-stimulated granuloma cells was similar to that produced by isolated intact granulomas. The ECF-producing activity of the cells was abolished by pretreatment with anti-Thy-1.2 or anti-Lyt-1.2 monoclonal antibody and complement but not by anti-Lyt-2.2 antibody. Furthermore, nylon wool-passed, T-enriched granuloma cells required collaboration of syngeneic macrophages to produce ECF. These results suggest that Lyt-1-positive T cells in the granuloma could, in collaboration with macrophages, produce ECF and thereby attract eosinophils to this lesion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Sasson S. Z., Shevach E., Green I., Paul W. E. Alloantiserum-induced inhibition of migration inhibition factor production in immune response gene-controlled immune systems. J Exp Med. 1974 Aug 1;140(2):383–395. doi: 10.1084/jem.140.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boros D. L., Warren K. S. Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J Exp Med. 1970 Sep 1;132(3):488–507. doi: 10.1084/jem.132.3.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheever A. W., Byram J. E., Hieny S., von Lichtenberg F., Lunde M. N., Sher A. Immunopathology of Schistosoma japonicum and S. mansoni infection in B cell depleted mice. Parasite Immunol. 1985 Jul;7(4):399–413. doi: 10.1111/j.1365-3024.1985.tb00086.x. [DOI] [PubMed] [Google Scholar]
  4. Cheever A. W., Byram J. E., von Lichtenberg F. Immunopathology of Schistosoma japonicum infection in athymic mice. Parasite Immunol. 1985 Jul;7(4):387–398. doi: 10.1111/j.1365-3024.1985.tb00085.x. [DOI] [PubMed] [Google Scholar]
  5. Doughty B. L., Phillips S. M. Delayed hypersensitivity granuloma formation and modulation around Schistosoma mansoni eggs in vitro. II. Regulatory T cell subsets. J Immunol. 1982 Jan;128(1):37–42. [PubMed] [Google Scholar]
  6. Garb K. S., Stavitsky A. B., Mahmoud A. A. Dynamics of antigen and mitogen-induced responses in murine schistosomiasis japonica: in vitro comparison between hepatic granulomas and splenic cells. J Immunol. 1981 Jul;127(1):115–120. [PubMed] [Google Scholar]
  7. Hirashima M., Tashiro K., Sakata K., Yoshimura T., Hayashi H. Difference in requirement of macrophage products for concanavalin A-induced production of eosinophil chemotactic factor and macrophage chemotactic factor from guinea pig lymphocytes in vitro. J Immunol. 1984 Oct;133(4):2012–2017. [PubMed] [Google Scholar]
  8. Ishii A., Owhashi M., Hashiguchi J. Schistosoma japonicum: immediate hypersensitivity reactions to egg antigen in humans and rodents. Exp Parasitol. 1982 Aug;54(1):104–112. doi: 10.1016/0014-4894(82)90116-3. [DOI] [PubMed] [Google Scholar]
  9. LITT M. Studies in experimental eosinophilia. V. Eosinophils in lynph nodes of guinea pigs following primary antigenic stimulation. Am J Pathol. 1963 May;42:529–549. [PMC free article] [PubMed] [Google Scholar]
  10. Ohta N., Minai M., Sasazuki T. Antigen-specific suppressor T lymphocytes (Leu-2a+3a-) in human schistosomiasis japonica. J Immunol. 1983 Nov;131(5):2524–2528. [PubMed] [Google Scholar]
  11. Owhashi M., Ishii A. Purification and characterization of a high molecular weight eosinophil chemotactic factor from Schistosoma japonicum eggs. J Immunol. 1982 Nov;129(5):2226–2231. [PubMed] [Google Scholar]
  12. Stavitsky A. B., Olds G. R., Peterson L. B. Regulation of egg antigen-induced in vitro proliferative response by splenic suppressor T cells in murine Schistosoma japonicum infection. Infect Immun. 1985 Sep;49(3):635–640. doi: 10.1128/iai.49.3.635-640.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wadee A. A., Sher R. The effects of a soluble factor released by sensitized mononuclear cells incubated with S. haematobium ova on eosinophil migration. Immunology. 1980 Dec;41(4):989–995. [PMC free article] [PubMed] [Google Scholar]
  14. Warren K. S., Boros D. L., Hang L. M., Mahmoud A. A. The Schistosoma japonicum egg granuloma. Am J Pathol. 1975 Aug;80(2):279–294. [PMC free article] [PubMed] [Google Scholar]
  15. Warren K. S., Domingo E. O. Granuloma formation around Schistosoma mansoni, S. HAEMATOBIUM, AND S. japonicum eggs. Size and rate of development, cellular composition, cross-sensitivity, and rate of egg destruction. Am J Trop Med Hyg. 1970 Mar;19(2):292–304. doi: 10.4269/ajtmh.1970.19.292. [DOI] [PubMed] [Google Scholar]
  16. Warren K. S., Grove D. I., Pelley R. P. The Schistosoma japonicum egg granuloma. II. Cellular composition, granuloma size, and immunologic concomitants. Am J Trop Med Hyg. 1978 Mar;27(2 Pt 1):271–275. doi: 10.4269/ajtmh.1978.27.271. [DOI] [PubMed] [Google Scholar]
  17. Wyler D. J., Postlethwaite A. E. Fibroblast stimulation in schistosomiasis. IV. Isolated egg granulomas elaborate a fibroblast chemoattractant in vitro. J Immunol. 1983 Mar;130(3):1371–1375. [PubMed] [Google Scholar]
  18. Wyler D. J., Wahl S. M., Cheever A. W., Wahl L. M. Fibroblast stimulation in schistosomiasis. I. Stimulation in vitro of fibroblasts by soluble products of egg granulomas. J Infect Dis. 1981 Sep;144(3):254–262. doi: 10.1093/infdis/144.3.254. [DOI] [PubMed] [Google Scholar]

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