Table 4.
The presence of MK1068 and MK1241 in the normal strain of H. magnanima that was injected with the purified fraction or PBS during the fifth instar. (Fifth-instar larvae of the normal strain were injected with the purified fraction or PBS. The injected larvae were reared to adults. Female adults were allowed to mate with uninjected normal strain males and to lay eggs. Ten egg masses were randomly collected, and the progeny of each were reared to adults. Total RNA was extracted from adults and subjected to gene-specific RT-PCR to detect MK1068 and MK1241. n.t., not tested.)
| (a) the adults from larvae that were injected with the purified fraction or PBS | ||||||
|---|---|---|---|---|---|---|
| inoculum | sex | no. of samples examined | no. of samples having | |||
| MK1068 | MK1241 | |||||
| the purified fraction | female | 5 | 5 | 5 | ||
| male | 4 | 4 | 4 | |||
| PBS | female | 5 | 0 | 0 | ||
| male | 5 | 0 | 0 | |||
| (b) the second-generation adults | ||||||
|---|---|---|---|---|---|---|
| egg mass no. | no. of progeny | sex ratio (proportion female) | MK1068 amplificationa | MK1241 amplificationa | ||
| female | male | female | male | |||
| 1 | 98 | 0.39 | 0/5 | 0/5 | 0/5 | 0/5 |
| 2 | 55 | 0.85b | 5/5 | 3/5 | 5/5 | 2/5 |
| 3 | 26 | 1.00b | 4/4 | n.t. | 4/4 | n.t. |
| 4 | 60 | 0.47 | 0/4 | 0/5 | 0/4 | 0/5 |
| 5 | 31 | 0.89b | 5/5 | 4/4 | 5/5 | 4/4 |
| 6 | 14 | 0.78b | 5/5 | 4/4 | 5/5 | 4/4 |
| 7 | 54 | 0.65b | 1/5 | 1/5 | 1/5 | 3/5 |
| 8 | 24 | 0.88b | 5/5 | 3/3 | 5/5 | 3/3 |
| 9 | 43 | 0.95b | 4/5 | n.t. | 5/5 | n.t. |
| 10 | 67 | 0.54 | 0/5 | 0/5 | 0/5 | 0/5 |
a
Number of amplified samples/number of tested samples.
b
Sex ratios were significantly different from 0.5 (5% level, binominal test).